NMR-based metabolomics for the identification of predictive urinary metabolic biomarkers of workers exposed to welding fumes and volatile organic compounds
Keywords:metabolomic, urinary biomarker, welding
An untargeted urinary NMR-based metabolomic approach have been applied in order to evaluate the occupational exposure of workers to welding fumes and volatile organic compounds in a metal carpentry company. In addition, the data obtained in this study have been integrated with urinary datasets coming from targeted HPLC-MS/MS and ICP-MS analyses for the quantification of oxidative stress biomarkers and metals, respectively. NMR quantified 35 metabolites and multivariate statistical analysis was then applied to the integrated dataset, identifying the most important variables for the discrimination of classes (welding fumes and volatile organic compounds exposed workers). In particular, methylguanidine (MG), pseudouridine (PSI), 8-oxo-7,8-dihydroguanosine (8-OxoGuo) and mercury (Hg) have been identified as possible predictive biomarkers of welding fumes exposure, whereas 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-OxodGuo) could be associated to volatile organic compounds exposure.
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